2. Anti-infection
  3. Bacterial Antibiotic
  4. Spectinomycin dihydrochloride

Spectinomycin dihydrochloride  (Synonyms: 盐酸大观霉素)

目录号: HY-B0438 纯度: 99.61%
COA 产品使用指南

摩尔计算器

Spectinomycin dihydrochloride 是一种广谱抗生素，可以抑制多种革兰氏阳性和革兰氏阴性细菌的生长。Spectinomycin dihydrochloride 通过特异靶向细菌核糖体，干扰蛋白质合成。Spectinomycin dihydrochloride 也是 td 内含子 RNA 的非竞争性抑制剂，Ki 为 7.2 mM。

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Spectinomycin dihydrochloride Chemical Structure

Spectinomycin dihydrochloride Chemical Structure

CAS No. : 21736-83-4

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规格 价格 是否有货 数量
10 mM * 1 mL in Water ￥550
In-stock
500 mg ￥494
In-stock
1 g ￥600
In-stock
5 g ￥950
In-stock
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50 g   询价  

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Other Forms of Spectinomycin dihydrochloride:

Spectinomycin dihydrochloride 相关抗体

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  • 生物活性

  • 纯度 & 产品资料

  • 参考文献

生物活性

Spectinomycin dihydrochloride is a broad-spectrum antibiotic and inhibits the growth of a variety of gram-positive and gram-negative organisms. Spectinomycin dihydrochloride acts by selectively targeting to the bacterial ribosome and interrupting protein synthesis. Spectinomycin dihydrochloride is also a noncompetitive inhibitor of td intron RNA with an Ki value of 7.2 mM[1]-[5].

IC50 & Target

bacterial ribosomal subunit[5]
体外研究
(In Vitro)

Spectinomycin dihydrochloride selectively inhibits protein synthesis in cells and in extracts of Escherichia coli:
Spectinomycin dihydrochloride (50 μg/mL) inhibits Escherichia coli growth rapidly and reversibly, and suppresses amino acid incorporation immediately[1].
Spectinomycin dihydrochloride (1 μg/mL or 3 μM) inhibits polypeptide synthesis directed either by endogenous messenger RNA or by MS-2 bacteriophage RNA, with maximum inhibition of 70-80% in extracts of Escherichia coli[1][1].
Spectinomycin dihydrochloride blocks the translocation of peptidyl-tRNAs from A-site to P-site by inhibiting the binding of elongation factor G to the ribosome[2].
Spectinomycin dihydrochloride interacts specifically with the residues G1064 and 01192 in 16S rRNA and potentially makes it inactive[2].
Spectinomycin dihydrochloride exhibits splicing inhibition and dependent on pH changes and Mg2+ concentration, indicating electrostatic interactions with the intron RNA[3].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Spectinomycin dihydrochloride 相关抗体:
体内研究
(In Vivo)

Spectinomycin dihydrochloride (20 mg/kg; i.m.; 20-100 mg/kg; 9 d) shows the safety in healthy chicks[4].
Spectinomycin dihydrochloride (10 mg/kg; i.v.; single dose) has the major elimination pathway by renal excretion, approximately 55% is excreted into the urine in unchanged form[5].
Pharmacokinetics of Spectinomycin dihydrochloride in Rat[5]

Parameter C0 (μg/mL) AUC0-∞ (μg•h/mL) Vd (L/kg) CL (L/h/kg) MRT (h) T1/2α (h) T1/2β (h) T1/2γ (h) fe CLrenal (L/h/kg) Eratio
Non atrioventricular analysis 44.3 16.8 0.756 0.602 0.757 / / / 0.553 0.359 1.00
Three-compartment model 37.8 15.7 0.747 0.649 1.11 / 0.237 0.754 19.5 / /

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Arbor Acres plus broiler chicks (15-day-old)[4]
Dosage: 20 mg/kg, 60 mg/kg, 100 mg/kg
Administration: Intramuscular injection (chest muscles); 9 days
Result: Showed biosecurity of 20 mg/kg by complete blood count, biochemical parameters, histopathological, clinical signs, body weight gain, and feed conversion ratio (FCR).
Resulted minor toxicity of 60 mg/kg.
分子量

405.27

Formula

C14H26Cl2N2O7
CAS 号
性状

固体

颜色

White to off-white

中文名称

盐酸大观霉素
结构分类
初始来源

bacterium Streptomyces spectabilis
运输条件

Room temperature in continental US; may vary elsewhere.
储存方式

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

溶解性数据
细胞实验： 

H2O 中的溶解度 : ≥ 100 mg/mL (246.75 mM)

* "≥" means soluble, but saturation unknown.

配制储备液
浓度 溶剂体积 质量 1 mg 5 mg 10 mg
1 mM 2.4675 mL 12.3375 mL 24.6749 mL
5 mM 0.4935 mL 2.4675 mL 4.9350 mL
查看完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

  • 摩尔计算器

  • 稀释计算器

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

质量
=
浓度
×
体积
×
分子量 *

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

浓度 (start)

C1

×
体积 (start)

V1

=
浓度 (final)

C2

×
体积 (final)

V2

动物实验：

以下溶解方案，请直接配制工作液。建议现用现配，在短期内尽快用完。 以下溶剂前显示的百分比是指该溶剂在您配制终溶液中的体积占比； 如在配制过程中出现沉淀、析出现象，可以通过加热和/或超声的方式助溶。

  • 方案 一

    请依序添加每种溶剂： PBS

    Solubility: 50 mg/mL (123.37 mM); 澄清溶液; 超声助溶

动物溶解方案计算器
请输入动物实验的基本信息：

给药剂量

mg/kg

动物的平均体重

g

每只动物的给药体积

μL

动物数量

只
由于实验过程有损耗，建议您多配一只动物的量
计算结果
工作液所需浓度 : mg/mL
该产品水溶性佳，请具体参考实测 水 / PBS / Saline 中的溶解度数据。
您所需的储备液浓度超过该产品的实测溶解度，如有需要，请与 MCE 中国技术支持联系。
纯度 & 产品资料

纯度: 99.61%

COA (273 KB) HNMR (255 KB) RP-HPLC (155 KB)

产品使用指南 (1538 KB)
参考文献

Spectinomycin dihydrochloride 相关分类

完整储备液配制表

* 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。
储备液的保存方式和期限：-80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)。-80°C储存时，请在6个月内使用，-20°C储存时，请在1个月内使用。

可选溶剂 浓度 溶剂体积 质量 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 2.4675 mL 12.3375 mL 24.6749 mL 61.6873 mL
5 mM 0.4935 mL 2.4675 mL 4.9350 mL 12.3375 mL
10 mM 0.2467 mL 1.2337 mL 2.4675 mL 6.1687 mL
15 mM 0.1645 mL 0.8225 mL 1.6450 mL 4.1125 mL
20 mM 0.1234 mL 0.6169 mL 1.2337 mL 3.0844 mL
25 mM 0.0987 mL 0.4935 mL 0.9870 mL 2.4675 mL
30 mM 0.0822 mL 0.4112 mL 0.8225 mL 2.0562 mL
40 mM 0.0617 mL 0.3084 mL 0.6169 mL 1.5422 mL
50 mM 0.0493 mL 0.2467 mL 0.4935 mL 1.2337 mL
60 mM 0.0411 mL 0.2056 mL 0.4112 mL 1.0281 mL
80 mM 0.0308 mL 0.1542 mL 0.3084 mL 0.7711 mL
100 mM 0.0247 mL 0.1234 mL 0.2467 mL 0.6169 mL

* 备注：如您选择水作为储备液，请稀释至工作液后，再用 0.22 μm 的滤膜过滤除菌后使用。

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Spectinomycin21736-83-4盐酸大观霉素BacterialAntibioticbiosecurityaminocyclitolbroad-spectrumgram-positivegram-negativeorganismbacterial ribosomeEscherichia coliamino acid incorporationMS-2 bacteriophagepeptidyl-tRNAelectrostatic interactionInhibitorinhibitorinhibit

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